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|Title:||Evaluation of the population heterogeneity of TBEV laboratory variants using high-throughput sequencing||Authors:||Litov, Alexander G
Deviatkin, Andrey A
Goptar, Irina A
Gmyl, Anatoly P
Markelov, Mikhail L
Shipulin, German A
Karganova, Galina G
Dedkov, Vladimir G.
|Keywords:||HTS;NGS;SNV;TBEV;molecular memory;population||Issue Date:||Feb-2018||Publisher:||Microbiology Society||Journal:||Journal of General Virology||Abstract:||We studied minor variants within two tick-borne encephalitis virus (TBEV) populations with a common ancestor: the mouse brain-adapted variant EK-328c and the tick-adapted variant M. High-throughput sequencing with custom amplicons from RT-PCR viral RNA was performed on Illumina MiSeq 2*250 paired-end v2 chemistry. Using the LowFreq program (default settings) and Sanger-sequenced consensus as a reference, variants with an abundance of 1 % and above within the studied populations were identified. Using the obtained data in the context of our previous studies, we concluded that TBEV variants, which are different from the major population phenotype and can become a major part of the viral population under favourable environmental conditions, can exist at abundances of less than 1 % in the long-term. The comparison of our data with the literature allowed us to conclude that the laboratory variant EK-328c and variant M have similar SNV counts to TBEV variants from natural populations and some fast-evolving RNA viruses.||URI:||https://cris.pasteurorg.ru/handle/123456789/24||ISSN:||0022-1317||DOI:||10.1099/jgv.0.001003|
|Appears in Collections:||Journal articles|
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