Please use this identifier to cite or link to this item:
Title: Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA
Authors: Zatsepin, Timofei S
Varizhuk, Anna M
Shipulin, German A
Aralov, Andrey V
Dedkov, Vladimir G. 
Keywords: G-clamp;Modified primer;Oligonucleotide;RT-qPCR;dsRNA
Issue Date: 2019
Publisher: Humana Press
Journal: Methods in Molecular Biology 
Abstract: We developed a new technique suitable for improved detection of low-copy dsRNA using modified oligonucleotides as primers in RT-qPCR. Insertion of G8AE-clamp residues into primers significantly improves thermal stability of duplexes with RNA without decrease of hybridization selectivity. The applicability of modified primers is demonstrated for detection of low-copy Kemerovo virus dsRNA.
ISBN: 978-1-4939-9215-7
ISSN: 1064-3745
DOI: 978-1-4939-9215-7
Appears in Collections:Journal articles

Show full item record

Page view(s)

checked on Jul 18, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.