Please use this identifier to cite or link to this item: https://cris.pasteurorg.ru/handle/123456789/27
Title: Photophysical properties and in vitro photocytotoxicity of disodium salt 2.4-di(alpha-methoxyethyl)-deuteroporphyrin-IX (Dimegine)
Authors: Dadeko, A V
Lilge, L
Kaspler, P
Murav'eva, T D
Starodubtcev, A M
Kiselev, V M
Zarubaev, V V
Ponomarev, G V
Keywords: Dimegine;cell accumulation;fluorescence quantum yield;photobleaching coefficient;photocytotoxicity;photosensitizer;singlet oxygen quantum yield
Issue Date: 6-Nov-2018
Publisher: Elsevier
Journal: Photodiagnosis and Photodynamic Therapy 
Abstract: Photophysical and in vitro photocytotoxicity studies were performed for the photosensitizer Dimegine, a disodium salt 2.4-di(alpha-methoxyethyl)-deuteroporphyrin-IX with very low systemic toxicity. The singlet oxygen and luminescence quantum yield were ΦΔ = 0,65 ± 0,06, and Φƒ=0,11 ± 0,01 respectively, and were independent of the excitation wavelength. The photobleaching coefficients for Dimegine dissolved in phosphate buffer (pH 7.4), and DMEM medium at concentration 2 μM/l and in phosphate buffer (pH 7.0) at concentration 10 μM/l were 16·10-5, 9·10-5 and 2·10-5 respectively. In vitro cellular distribution and photocytotoxicity was studied in two human (U87 - primary glioblastoma and HT1376 - bladder cancer) and two rat cell lines (RG2 - glioma, and AY27 - bladder carcinoma). Fluorescence microscopy analysis shows primary Dimegine accumulation as small fluorescent inclusion bodies around the nuclei, suggesting an apoptotic over a necrotic cell death mechanism. The PDT efficacy was slightly higher for the rat cell lines over the human-derived cell lines, with LD50 values of 2,5 μM/l, 2.8 μM/l, 4.5 μM/l, 2.8 μM/l using 530 nm excitation wavelength for AY27, RG2, HT1376 and U87 respectively, and 1.8 μM/l, 2 μM/l, 5 μM/l, 2.4 μM/l using 625 nm excitation wavelength for AY27, RG2, HT1376 and U87 respectively. Comparison to literature data showed that Dimegine demonstrated improved phototherapeutic characteristics comparing to PpIX-mediated PDT.
URI: https://cris.pasteurorg.ru/handle/123456789/27
ISSN: 1572-1000
DOI: 10.1016/j.pdpdt.2018.11.006
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